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In vitro recombination
Recombinant DNA (rDNA), or molecular cloning, is the process by which a single gene, or segment of DNA, is isolated and amplified. Recombinant DNA is also known as in vitro recombination.
A cloning vector is a DNA molecule that carries foreign DNA into a host cell, where it replicates, producing many copies of itself along with the foreign DNA. There are many types of cloning vectors such plasmid, phage. In order to carry out recombination between vector and the foreign DNA, it is necessary the vector and DNA to be cloned by digestion, ligase the foreign DNA into the vector with the enzyme DNA ligase. And DNA is inserted by introducing the DNA into bacteria cells by transformation.
File:Recombinant formation of plasmids.svg|Recombination DNA( Molecular Cloning)
== Preparation of foreign DNA ==
There are two major sources of foreign DNA for molecular cloning is genomic DNA (gDNA) and complementary (or copy) DNA (cDNA). cDNA molecules are DNA copies of mRNA molecules, produced in vitro by action of the enzyme reverse transcriptase. In order to obtain the cDNA for a specific gene, it is first necessary to construct a cDNA library.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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